actinomycetemcomitans colonization or response to serotypes and the immunoglobulin G response to CDT subunits. actinomycetemcomitans correlated with subgingival colonization (R = 0.75, p < 0.05). A response to serotype b was detected in localized (66.7%) and generalized aggressive periodontitis (54.5%). Sera from 75.0% localized and 81.8% generalized aggressive periodontitis patients reacted to A.
actinomycetemcomitans subgingival levels and neutralization of CDT activity were also analyzed. actinomycetemcomitans serotypes a/b/c and to each CDT subunit (CdtA, CdtB and CdtC) by ELISA. Sera from periodontally healthy, localized and generalized aggressive periodontitis and chronic periodontitis subjects (n = 80) were assessed for immunoglobulin G titers to A. actinomycetemcomitans would lead to a response against CDT. This study aimed to test whether subgingival colonization and humoral response to A. In spite of its association with pathogenesis, little is known about the humoral immune response against the CDT. periodontitis), they may not be specific markers for chancroid infection.Ĭytolethal distending toxin (CDT) is a genotoxin produced by Aggregatibacter actinomycetemcomitans. The data suggest that the low levels of anti-HdCDT antibodies, which include neutralizing antibodies, may contribute to limited protection in chancroid and since anti-HdCDT antibodies, may be detected in healthy individuals and in patients with certain disease conditions (e.g. High levels of neutralizing antibodies (> or =160) were detected in only 22 and 2% of the patients with chancroid and periodontitis, respectively. Antibodies to CdtC, being less frequently detected than the antibodies to other components, show a good correlation with the neutralizing capacity of sera. In the sera of the 50 patients with chancroid, antibodies to CdtA, CdtB, and CdtC were detected in 50, 35, and 34 individuals, respectively.
However, high levels (> or =100 units) of antibodies to HdCDT complex were significantly more prevalent in the sera of patients with both chancroid and periodontitis than in the sera of the corresponding controls (P=0.001 and P=0.04, respectively). Our results demonstrate that majority of chancroid patients and healthy individuals had detectable levels of serum antibodies to HdCDT complex and to separate toxin components.
neutralization capacity of the sera were tested. In addition, the biological activity e.g. Antibodies specific for the cytolethal-distending toxin of Haemophilus ducreyi (HdCDT) complex and for the CdtA, CdtB, and CdtC components were measured by ELISA in the sera of 50 patients with culture and/or PCR proven chancroid, 42 patients with periodontitis, 50 blood donors from Tanzania, 50 blood donors from Sweden.
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